國立高雄大學圖資館 |

Biocontrol of plant diseases by bacillus subtilis :basic and practical applications /

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Biocontrol of plant diseases by bacillus subtilis :Makoto Shoda.
Reminder of title:	basic and practical applications /
Author:	Shoda, Makoto,
Description:	xxiii, 325 pages :illustrations ;25 cm.
Subject:	Microbial pesticides.
ISBN:	9780367136109

Biocontrol of plant diseases by bacillus subtilis :basic and practical applications /
Shoda, Makoto,

Biocontrol of plant diseases by bacillus subtilis :basic and practical applications /Makoto Shoda. - xxiii, 325 pages :illustrations ;25 cm. - New directions in organic and biological chemistry.

Includes bibliographical references and index.

Note continued: 6.1.2.2.Disruption of the ituD Gene to Derive the Iturin A-Deficient Phenotype -- 6.1.2.3.Promoter Analysis of the ituD Gene -- 6.1.2.4.Promoter Replacement of the Iturin A Operon for High Iturin A Production -- 6.1.3.Discussion -- 6.2.Conversion of B. subtilis 168 into an Iturin Producer by Transfer of Iturin A Operon -- 6.2.1.Materials and Methods -- 6.2.1.1.Bacterial Strains, Phage, Plasmids, and Media -- 6.2.1.2.Transformation and DNA Manipulation -- 6.2.1.3.Construction of LPA -- 6.2.1.4.Preparation of a Recipient Strain Containing the LPA -- 6.2.1.5.Iturin Operon Transfer -- 6.2.1.6.Introduction of sfp and degQ -- 6.2.1.7.Quantitative Analysis of Iturin A, Plipastatin, and Surfactin -- 6.2.2.Results -- 6.2.2.1.Horizontal Transfer of Iturin A Synthetase Operon to Strain 168 -- 6.2.2.2.Conversion of Iturin A Operon-Transferred Strain into an Iturin A Producer -- 6.2.3.Discussion -- 6.3.Genetic Analysis for Conversion of B. subtilis 168 to Plipastatin Production -- 6.3.1.Materials and Methods -- 6.3.1.1.Strains and Media -- 6.3.1.2.Purification of Plipastatins -- 6.3.1.3.HPLC Analysis of Plipastatin -- 6.3.1.4.Mass Spectrometry and NMR -- 6.3.1.5.Transformation and DNA Manipulation -- 6.3.1.6.Transposon Mutagenesis -- 6.3.1.7.Southern Blot Analysis -- 6.3.1.8.Construction of degQ-Related Mutants -- 6.3.1.9.DNA Sequencing of the degQ Region -- 6.3.1.10.Nucleotide Sequence Accession Number -- 6.3.2.Results -- 6.3.2.1.Conversion of Strain MI113 into a Plipastatin Producer -- 6.3.2.2.Transposon Mutagenesis of Strain 406 -- 6.3.2.3.Isolation and Identification of the Antifungal Antibiotic Produced by Strain 406 -- 6.3.2.4.Identification of Notl Fragment with Mini-Tn10 Inserted -- 6.3.2.5.Inactivation of Plipastatin Synthetase Gene with pNEXT24 and pNEXT44 -- 6.3.2.6.Production of Plipastatin by Strains Carrying lpa-8 -- 6.3.2.7.Role of degQ of YB8 in Plipastatin Production -- 6.3.3.Discussion -- References -- 7.1.Production of Iturin by Foam Separation in Submerged Fermentation -- 7.1.1.Materials and Methods -- 7.1.1.1.Strain and Medium Used -- 7.1.1.2.Operation of Jar Fermentor -- 7.1.1.3.Assay of Iturin -- 7.1.1.4.Cell and Sugar Concentrations -- 7.1.2.Results -- 7.1.2.1.Effect of Aeration Rate -- 7.1.2.2.Effect of Temperature -- 7.1.2.3.Effect of Glucose and Polypepton Concentrations -- 7.1.2.4.Introduction of a New Basket-Type Agitation Unit -- 7.2.Production of Iturin in Solid-State Fermentation -- 7.2.1.Materials and Methods -- 7.2.1.1.Preservation of B. subtilis NB22 -- 7.2.1.2.Preparation of Liquid Inoculum of B. subtilis NB22 for SSF at 15 g Scale -- 7.2.1.3.Preparation of Okara as a Solid Substrate -- 7.2.1.4.SSF at 15 g Scale -- 7.2.1.5.Sampling for the Measurement of pH, Cell Population, and Water Content -- 7.2.1.6.Extraction and Measurement of Iturin -- 7.2.1.7.Suspension Fermentation (SF) of Okara -- 7.2.1.8.SSF at 300-500 g Scale -- 7.2.1.9.Sampling Position -- 7.2.1.10.SSF at 3.0 kg Scale -- 7.2.1.11.Sampling -- 7.2.1.12.Measurement of Temperature -- 7.2.1.13.Measurement of pH, Cell Concentration, Iturin, and Water Content -- 7.2.2.Results -- 7.2.2.1.15 g Scale SSF -- 7.2.2.2.Static 300-500 g SSF -- 7.2.2.3.Aerated 300-500 g SSF -- 7.2.2.4.3 kg Scale SSF -- 7.2.2.5.Comparison of Iturin Production in SmF and SSF -- 7.3.Solid-State Fermentation Using Dehydrated Material -- 7.3.1.Materials and Methods -- 7.3.1.1.Microorganism Strain -- 7.3.1.2.Preparation of Seeding Culture -- 7.3.1.3.Preparation of Okara as a Solid Substrate -- 7.3.1.4.Partially Dehydrated Okara -- 7.3.1.5.Measurement of Viable Cells -- 7.3.1.6.Temperature Dependency of Iturin A Production -- 7.3.1.7.Extraction and Measurement of Iturin A -- 7.3.2.Results and Discussion -- 7.3.2.1.Measurement of Viable Cells -- 7.3.2.2.Optimal Temperature for Iturin A Production -- 7.3.2.3.Temperature Distribution in the 3 kg Scale Reactor -- 7.3.2.4.Iturin A Production in Intact Okara -- 7.3.2.5.Effect of Moisture Content on Iturin A Production by B. subtilis NB22 -- 7.4.Dual Production of Iturin and Surfactin in Solid-State Fermentation -- 7.4.1.Materials and Methods -- 7.4.1.1.Strain Used -- 7.4.1.2.Solid-State Fermentation of Okara -- 7.4.1.3.Measurement of Iturin and Surfactin Concentrations -- 7.4.2.Results -- 7.4.2.1.Effect of Temperature on Production of Iturin and Surfactin -- 7.4.2.2.Change in the Ratio of the Iturin Homologues at Different Temperatures -- 7.5.Enhanced Iturin Production in Submerged Fermentation and Plant Test -- 7.5.1.Materials and Methods -- 7.5.1.1.Microorganisms -- 7.5.1.2.Media -- 7.5.1.3.Cultivation of RB14-CS -- 7.5.1.4.Quantitative Analysis of Iturin A in Liquid Medium -- 7.5.1.5.Elementary Analysis of the Water- Soluble Fraction -- 7.5.1.6.Inoculation of R. solani into Soil -- 7.5.1.7.Effects of RB14-CS on the Plant Growth in Soil -- 7.5.1.8.Cell Recovery from Soil and Determination of Cell Number -- 7.5.1.9.Quantitative Analysis of Iturin A Recovered from Soil -- 7.5.1.10.Statistical Analysis -- 7.5.2.Results -- 7.5.2.1.Enhancement of Iturin A Production by Changing and Increasing Nitrogen and Carbon Sources in Media -- 7.5.2.2.Carbon and Nitrogen Contents in the Water-Soluble Portions of the Media -- 7.5.2.3.Suppressive Effects of Cultures Grown in SM and no. 3S Media in Plant Tests -- 7.5.3.Discussion -- 7.6.Enhanced Iturin Production in Solid-State Fermentation -- 7.6.1.Materials and Methods -- 7.6.1.1.Strains -- 7.6.1.2.Media Used -- 7.6.1.3.Solid-State Fermentation -- 7.6.1.4.Determination of Viable Cell Number and pH -- 7.6.1.5.Extraction and Quantitation of Iturin A -- 7.6.1.6.Soil Used -- 7.6.1.7.Plant Test -- 7.6.1.8.Fertilizer Effect of Cultured Okara -- 7.6.1.9.Quantitative Analysis of Iturin A Recovered from Soil -- 7.6.1.10.Fertilizer Analysis -- 7.6.1.11.Statistical Analysis -- 7.6.2.Results -- 7.6.2.1.Iturin A Production by B. subtilis RB 14-CS in Solid-State Fermentation -- 7.6.2.2.Suppressive Effects of Okara Cultivated with RB14-CS on Damping-Off of Tomato -- 7.6.2.3.Viable Cell Number and Iturin A Concentration in Soil -- 7.6.2.4.Fertilizer Effects of Okara Cultured with RB14-CS on the Growth of Tomato -- 7.6.2.5.Comparison of Iturin A Production in Solid-State Fermentation and Submerged Fermentation -- 7.6.3.Discussion -- 7.7.Optimization of Iturin Production in Solid-State Fermentation -- 7.7.1.Materials and Methods -- 7.7.1.1.Strains -- 7.7.1.2.Preparation of Seeding Culture -- 7.7.1.3.Solid-State Fermentation -- 7.7.1.4.Extraction and Measurement of Iturin A -- 7.7.1.5.Selection of Carbon and Nitrogen Sources -- 7.7.1.6.Measurement of Reducing Sugar Concentration -- 7.7.1.7.Experimental Design -- 7.7.1.8.Determination of Viable Cell Number -- 7.7.1.9.Disease Suppression in Tomato Seedlings -- 7.7.1.10.Statistical Analysis -- 7.7.2.Results -- 7.7.2.1.Factors Affecting the Production of Iturin A in the SSF -- 7.7.2.2.Medium Optimization Using Response Surface Methodology -- 7.7.2.3.Suppressive Effects of the Optimum SSF Product on the Plant Disease -- 7.7.3.Discussion -- 7.8.Association of Biofilm Formation with Iturin Production -- 7.8.1.Materials and Methods -- 7.8.1.1.Strain -- 7.8.1.2.Preparation of Seed Culture -- 7.8.1.3.Solid-State Fermentation and Submerged Fermentation Using Soybean Curd Residue, Okara, as a Substrate -- 7.8.1.4.Extraction and Quantitation of Iturin A from SSF and SmF -- 7.8.1.5.Biofilm Fermentation in the AMS Reactor and Submerged Fermentation Using No. 3S Medium -- 7.8.1.6.Scanning Electron Microscopy (SEM) -- 7.8.1.7.Determination of Growth and Iturin A Production in the AMS Reactor and SmF -- 7.8.1.8.Whole-Cell Protein Extraction for Proteome Analysis -- 7.8.1.9.Two-Dimensional Electrophoresis -- 7.8.1.10.Analysis of Proteome Patterns -- 7.8.1.11.N-Terminal Amino Acid Sequencing and Protein Identifications -- 7.8.1.12.RNA Isolation and Real-Time RT-PCR -- 7.8.2.Results -- 7.8.2.1.Comparison of Iturin A Production between SSF and SmF Using Soybean Curd Residue, Okara, as a Substrate -- 7.8.2.2.Growth and Iturin A Production by Biofilm Fermentation in an AMS Reactor and in SmF Using No. 3S Liquid Medium -- 7.8.2.3.Proteome Analysis and Identification of Differentially Expressed Proteins -- 7.8.2.4.Real-Time RT-PCR -- 7.8.2.5.Scanning Electron Microscopy -- 7.8.3.Discussion -- 7.9.Maximization of High Spore Density in Liquid Cultivation by B. subtilis RB14 -- 7.9.1.Materials and Methods -- 7.9.1.1.Strain Used -- 7.9.1.2.Reactor -- 7.9.1.3.Medium -- 7.9.1.4.Operation Condition -- 7.9.1.5.Cell Concentration -- 7.9.1.6.Oxygen Supply -- 7.9.2.Results -- 7.9.2.1.The Effect of Metal Salts -- 7.9.2.2.Effect of Other Factors -- 7.9.2.3.Maximizing Operation of Spore -- References -- 8.1.Surfactin Production by a Recombinant B. subtilis in Liquid Cultivation -- 8.1.1.Materials and Methods -- 8.1.1.1.Strain and Plasmid -- 8.1.1.2.Cultivation of B. subtilis MI113(pC112) -- 8.1.1.3.Measurement of Cell Concentration -- 8.1.1.4.Evaluation of Plasmid Stability -- 8.1.1.5.Extraction and Measurement of Surfactin and Iturin -- 8.1.2.Results -- 8.1.2.1.Time Course of Surfactin Production by B. subtilis MI113(pC112) and RB14 -- 8.1.2.2.Time Course of Cell Concentration -- 8.1.2.3.Plasmid Stability -- 8.2.Surfactin Production and Plasmid Stability in Solid-State Fermentation (SSF) -- 8.2.1.Materials and Methods -- 8.2.1.1.Strains and Recombinant Plasmid Used -- 8.2.1.2.Preparation of Seeding Culture -- 8.2.1.3.Submerged Fermentation (SmF) in No. 3S Medium Modified to 3% Polypepton S -- 8.2.1.4.Solid-State Fermentation -- 8.2.1.5.Measurement of Viable Cells -- 8.2.1.6.Evaluation of Plasmid Stability -- 8.2.1.7.Extraction and Measurement of Surfactin -- 8.2.1.8.Estimation of the Soluble Nutrients -- 8.2.2.Results -- 8.2.2.1.Effect of Moisture Content on Surfactin Production by B. subtilis MI113(pC112) -- 8.2.2.2.Effect of Temperature on Surfactin Production by B. subtilis MI113(pC112) and RB14

"Plant diseases are a serious threat to food production. This unique volume provides the fundamental knowledge and practical use of B.subtilis as a promising biocontrol agent. In order to replace chemical pesticides, one possibility is microbial pesticides using safe microbes. Bacillus subtilis is one of several candidates. Screening of the bacterium, the application of plant tests, clarification of its suppressive mechanism to plant pathogens and engineering aspects of suppressive peptides production are presented here. The author illustrates how B. subtilis is far more advantageous than, for example, Pseudomonas in biocontrol and can be considered as an useful candidate"--

ISBN: 9780367136109

LCCN: 2019030557

Nat. Bib. No.: GBB9F5917bnb

Nat. Bib. Agency Control No.: 019540474UkSubjects--Topical Terms:

337744
Microbial pesticides.

LC Class. No.: SB976.M55 / S56 2020

Dewey Class. No.: 632/.96

Biocontrol of plant diseases by bacillus subtilis :basic and practical applications /
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505 0 $a Note continued: 6.1.2.2.Disruption of the ituD Gene to Derive the Iturin A-Deficient Phenotype -- 6.1.2.3.Promoter Analysis of the ituD Gene -- 6.1.2.4.Promoter Replacement of the Iturin A Operon for High Iturin A Production -- 6.1.3.Discussion -- 6.2.Conversion of B. subtilis 168 into an Iturin Producer by Transfer of Iturin A Operon -- 6.2.1.Materials and Methods -- 6.2.1.1.Bacterial Strains, Phage, Plasmids, and Media -- 6.2.1.2.Transformation and DNA Manipulation -- 6.2.1.3.Construction of LPA -- 6.2.1.4.Preparation of a Recipient Strain Containing the LPA -- 6.2.1.5.Iturin Operon Transfer -- 6.2.1.6.Introduction of sfp and degQ -- 6.2.1.7.Quantitative Analysis of Iturin A, Plipastatin, and Surfactin -- 6.2.2.Results -- 6.2.2.1.Horizontal Transfer of Iturin A Synthetase Operon to Strain 168 -- 6.2.2.2.Conversion of Iturin A Operon-Transferred Strain into an Iturin A Producer -- 6.2.3.Discussion -- 6.3.Genetic Analysis for Conversion of B. subtilis 168 to Plipastatin Production -- 6.3.1.Materials and Methods -- 6.3.1.1.Strains and Media -- 6.3.1.2.Purification of Plipastatins -- 6.3.1.3.HPLC Analysis of Plipastatin -- 6.3.1.4.Mass Spectrometry and NMR -- 6.3.1.5.Transformation and DNA Manipulation -- 6.3.1.6.Transposon Mutagenesis -- 6.3.1.7.Southern Blot Analysis -- 6.3.1.8.Construction of degQ-Related Mutants -- 6.3.1.9.DNA Sequencing of the degQ Region -- 6.3.1.10.Nucleotide Sequence Accession Number -- 6.3.2.Results -- 6.3.2.1.Conversion of Strain MI113 into a Plipastatin Producer -- 6.3.2.2.Transposon Mutagenesis of Strain 406 -- 6.3.2.3.Isolation and Identification of the Antifungal Antibiotic Produced by Strain 406 -- 6.3.2.4.Identification of Notl Fragment with Mini-Tn10 Inserted -- 6.3.2.5.Inactivation of Plipastatin Synthetase Gene with pNEXT24 and pNEXT44 -- 6.3.2.6.Production of Plipastatin by Strains Carrying lpa-8 -- 6.3.2.7.Role of degQ of YB8 in Plipastatin Production -- 6.3.3.Discussion -- References -- 7.1.Production of Iturin by Foam Separation in Submerged Fermentation -- 7.1.1.Materials and Methods -- 7.1.1.1.Strain and Medium Used -- 7.1.1.2.Operation of Jar Fermentor -- 7.1.1.3.Assay of Iturin -- 7.1.1.4.Cell and Sugar Concentrations -- 7.1.2.Results -- 7.1.2.1.Effect of Aeration Rate -- 7.1.2.2.Effect of Temperature -- 7.1.2.3.Effect of Glucose and Polypepton Concentrations -- 7.1.2.4.Introduction of a New Basket-Type Agitation Unit -- 7.2.Production of Iturin in Solid-State Fermentation -- 7.2.1.Materials and Methods -- 7.2.1.1.Preservation of B. subtilis NB22 -- 7.2.1.2.Preparation of Liquid Inoculum of B. subtilis NB22 for SSF at 15 g Scale -- 7.2.1.3.Preparation of Okara as a Solid Substrate -- 7.2.1.4.SSF at 15 g Scale -- 7.2.1.5.Sampling for the Measurement of pH, Cell Population, and Water Content -- 7.2.1.6.Extraction and Measurement of Iturin -- 7.2.1.7.Suspension Fermentation (SF) of Okara -- 7.2.1.8.SSF at 300-500 g Scale -- 7.2.1.9.Sampling Position -- 7.2.1.10.SSF at 3.0 kg Scale -- 7.2.1.11.Sampling -- 7.2.1.12.Measurement of Temperature -- 7.2.1.13.Measurement of pH, Cell Concentration, Iturin, and Water Content -- 7.2.2.Results -- 7.2.2.1.15 g Scale SSF -- 7.2.2.2.Static 300-500 g SSF -- 7.2.2.3.Aerated 300-500 g SSF -- 7.2.2.4.3 kg Scale SSF -- 7.2.2.5.Comparison of Iturin Production in SmF and SSF -- 7.3.Solid-State Fermentation Using Dehydrated Material -- 7.3.1.Materials and Methods -- 7.3.1.1.Microorganism Strain -- 7.3.1.2.Preparation of Seeding Culture -- 7.3.1.3.Preparation of Okara as a Solid Substrate -- 7.3.1.4.Partially Dehydrated Okara -- 7.3.1.5.Measurement of Viable Cells -- 7.3.1.6.Temperature Dependency of Iturin A Production -- 7.3.1.7.Extraction and Measurement of Iturin A -- 7.3.2.Results and Discussion -- 7.3.2.1.Measurement of Viable Cells -- 7.3.2.2.Optimal Temperature for Iturin A Production -- 7.3.2.3.Temperature Distribution in the 3 kg Scale Reactor -- 7.3.2.4.Iturin A Production in Intact Okara -- 7.3.2.5.Effect of Moisture Content on Iturin A Production by B. subtilis NB22 -- 7.4.Dual Production of Iturin and Surfactin in Solid-State Fermentation -- 7.4.1.Materials and Methods -- 7.4.1.1.Strain Used -- 7.4.1.2.Solid-State Fermentation of Okara -- 7.4.1.3.Measurement of Iturin and Surfactin Concentrations -- 7.4.2.Results -- 7.4.2.1.Effect of Temperature on Production of Iturin and Surfactin -- 7.4.2.2.Change in the Ratio of the Iturin Homologues at Different Temperatures -- 7.5.Enhanced Iturin Production in Submerged Fermentation and Plant Test -- 7.5.1.Materials and Methods -- 7.5.1.1.Microorganisms -- 7.5.1.2.Media -- 7.5.1.3.Cultivation of RB14-CS -- 7.5.1.4.Quantitative Analysis of Iturin A in Liquid Medium -- 7.5.1.5.Elementary Analysis of the Water- Soluble Fraction -- 7.5.1.6.Inoculation of R. solani into Soil -- 7.5.1.7.Effects of RB14-CS on the Plant Growth in Soil -- 7.5.1.8.Cell Recovery from Soil and Determination of Cell Number -- 7.5.1.9.Quantitative Analysis of Iturin A Recovered from Soil -- 7.5.1.10.Statistical Analysis -- 7.5.2.Results -- 7.5.2.1.Enhancement of Iturin A Production by Changing and Increasing Nitrogen and Carbon Sources in Media -- 7.5.2.2.Carbon and Nitrogen Contents in the Water-Soluble Portions of the Media -- 7.5.2.3.Suppressive Effects of Cultures Grown in SM and no. 3S Media in Plant Tests -- 7.5.3.Discussion -- 7.6.Enhanced Iturin Production in Solid-State Fermentation -- 7.6.1.Materials and Methods -- 7.6.1.1.Strains -- 7.6.1.2.Media Used -- 7.6.1.3.Solid-State Fermentation -- 7.6.1.4.Determination of Viable Cell Number and pH -- 7.6.1.5.Extraction and Quantitation of Iturin A -- 7.6.1.6.Soil Used -- 7.6.1.7.Plant Test -- 7.6.1.8.Fertilizer Effect of Cultured Okara -- 7.6.1.9.Quantitative Analysis of Iturin A Recovered from Soil -- 7.6.1.10.Fertilizer Analysis -- 7.6.1.11.Statistical Analysis -- 7.6.2.Results -- 7.6.2.1.Iturin A Production by B. subtilis RB 14-CS in Solid-State Fermentation -- 7.6.2.2.Suppressive Effects of Okara Cultivated with RB14-CS on Damping-Off of Tomato -- 7.6.2.3.Viable Cell Number and Iturin A Concentration in Soil -- 7.6.2.4.Fertilizer Effects of Okara Cultured with RB14-CS on the Growth of Tomato -- 7.6.2.5.Comparison of Iturin A Production in Solid-State Fermentation and Submerged Fermentation -- 7.6.3.Discussion -- 7.7.Optimization of Iturin Production in Solid-State Fermentation -- 7.7.1.Materials and Methods -- 7.7.1.1.Strains -- 7.7.1.2.Preparation of Seeding Culture -- 7.7.1.3.Solid-State Fermentation -- 7.7.1.4.Extraction and Measurement of Iturin A -- 7.7.1.5.Selection of Carbon and Nitrogen Sources -- 7.7.1.6.Measurement of Reducing Sugar Concentration -- 7.7.1.7.Experimental Design -- 7.7.1.8.Determination of Viable Cell Number -- 7.7.1.9.Disease Suppression in Tomato Seedlings -- 7.7.1.10.Statistical Analysis -- 7.7.2.Results -- 7.7.2.1.Factors Affecting the Production of Iturin A in the SSF -- 7.7.2.2.Medium Optimization Using Response Surface Methodology -- 7.7.2.3.Suppressive Effects of the Optimum SSF Product on the Plant Disease -- 7.7.3.Discussion -- 7.8.Association of Biofilm Formation with Iturin Production -- 7.8.1.Materials and Methods -- 7.8.1.1.Strain -- 7.8.1.2.Preparation of Seed Culture -- 7.8.1.3.Solid-State Fermentation and Submerged Fermentation Using Soybean Curd Residue, Okara, as a Substrate -- 7.8.1.4.Extraction and Quantitation of Iturin A from SSF and SmF -- 7.8.1.5.Biofilm Fermentation in the AMS Reactor and Submerged Fermentation Using No. 3S Medium -- 7.8.1.6.Scanning Electron Microscopy (SEM) -- 7.8.1.7.Determination of Growth and Iturin A Production in the AMS Reactor and SmF -- 7.8.1.8.Whole-Cell Protein Extraction for Proteome Analysis -- 7.8.1.9.Two-Dimensional Electrophoresis -- 7.8.1.10.Analysis of Proteome Patterns -- 7.8.1.11.N-Terminal Amino Acid Sequencing and Protein Identifications -- 7.8.1.12.RNA Isolation and Real-Time RT-PCR -- 7.8.2.Results -- 7.8.2.1.Comparison of Iturin A Production between SSF and SmF Using Soybean Curd Residue, Okara, as a Substrate -- 7.8.2.2.Growth and Iturin A Production by Biofilm Fermentation in an AMS Reactor and in SmF Using No. 3S Liquid Medium -- 7.8.2.3.Proteome Analysis and Identification of Differentially Expressed Proteins -- 7.8.2.4.Real-Time RT-PCR -- 7.8.2.5.Scanning Electron Microscopy -- 7.8.3.Discussion -- 7.9.Maximization of High Spore Density in Liquid Cultivation by B. subtilis RB14 -- 7.9.1.Materials and Methods -- 7.9.1.1.Strain Used -- 7.9.1.2.Reactor -- 7.9.1.3.Medium -- 7.9.1.4.Operation Condition -- 7.9.1.5.Cell Concentration -- 7.9.1.6.Oxygen Supply -- 7.9.2.Results -- 7.9.2.1.The Effect of Metal Salts -- 7.9.2.2.Effect of Other Factors -- 7.9.2.3.Maximizing Operation of Spore -- References -- 8.1.Surfactin Production by a Recombinant B. subtilis in Liquid Cultivation -- 8.1.1.Materials and Methods -- 8.1.1.1.Strain and Plasmid -- 8.1.1.2.Cultivation of B. subtilis MI113(pC112) -- 8.1.1.3.Measurement of Cell Concentration -- 8.1.1.4.Evaluation of Plasmid Stability -- 8.1.1.5.Extraction and Measurement of Surfactin and Iturin -- 8.1.2.Results -- 8.1.2.1.Time Course of Surfactin Production by B. subtilis MI113(pC112) and RB14 -- 8.1.2.2.Time Course of Cell Concentration -- 8.1.2.3.Plasmid Stability -- 8.2.Surfactin Production and Plasmid Stability in Solid-State Fermentation (SSF) -- 8.2.1.Materials and Methods -- 8.2.1.1.Strains and Recombinant Plasmid Used -- 8.2.1.2.Preparation of Seeding Culture -- 8.2.1.3.Submerged Fermentation (SmF) in No. 3S Medium Modified to 3% Polypepton S -- 8.2.1.4.Solid-State Fermentation -- 8.2.1.5.Measurement of Viable Cells -- 8.2.1.6.Evaluation of Plasmid Stability -- 8.2.1.7.Extraction and Measurement of Surfactin -- 8.2.1.8.Estimation of the Soluble Nutrients -- 8.2.2.Results -- 8.2.2.1.Effect of Moisture Content on Surfactin Production by B. subtilis MI113(pC112) -- 8.2.2.2.Effect of Temperature on Surfactin Production by B. subtilis MI113(pC112) and RB14
505 0 $a Note continued: 8.2.2.3.Effect of Temperature on Viable Cell Count of B. subtilis MI113(pC112) and RB14 and Plasmid Stability in MI113(pC112) -- 8.2.3.Discussion -- 8.3.New Variants of Surfactin in Solid-State Fermentation by a Recombinant Bacillus subtilis -- 8.3.1.Materials and Methods -- 8.3.1.1.Strains and Medium Used -- 8.3.1.2.Extraction and Analysis of Surfactin Variants -- 8.3.2.Results -- 8.3.3.Discussion -- 8.4.Plasmid Stability in B. subtilis NB22 -- 8.4.1.Materials and Methods -- 8.4.1.1.Strain Used -- 8.4.2.Results -- 8.4.2.1.Stability of pC194 -- 8.4.2.2.Stability in Schaeffer's Medium -- 8.4.2.3.Stability of pUB110 -- 8.4.3.Discussion -- References -- 9.1.Materials and Methods -- 9.1.1.Strain and Medium Used -- 9.1.2.Chemical Pesticide Used -- 9.1.3.Growth of RB14-C on Agar Plates Containing Flutolanil -- 9.1.4.Growth of RB14-C and Productivity of Iturin A and Surfactin in Liquid Medium Containing Flutolanil -- 9.1.5.Suppressive Effect of the Mixture of a Solution Containing Iturin A and Surfactin and Flutolanil on the Growth of R. solani In Vitro -- 9.1.6.Plant Test -- 9.1.7.Preparation of RB14-C and Flutolanil for Plant Test -- 9.1.8.Cell Recovery from the Soil and Counting the Number of Viable Cells -- 9.1.9.Quantitative Analysis of Iturin A and Surfactin Recovered from Soil -- 9.2.Results and Discussion -- 9.2.1.Growth of RB14-C on Plate Containing Flutolanil and Productivity of Iturin A and Surfactin in Medium Containing Flutolanil -- 9.2.2.Comparison of Growth of R. solani on Plates Containing a Mixture of Iturin A and Surfactin from RB14-C and Flutolanil -- 9.2.3.Plant Test -- References -- 10.1.Materials and Methods -- 10.1.1.Microorganisms and Inoculum Production -- 10.1.2.Examination of Mutual Inhibition of RB14-C and BY in In Vitro Tests -- 10.1.3.Inhibition of R. solani in Microcosm Studies -- 10.1.4.Biocontrol of Rhizoctonia Damping-Off under Growth Chamber Conditions -- 10.1.5.Number of R. solani in Soil -- 10.1.6.Number of B. subtilis RB14-C in Soil -- 10.1.7.Statistical Analysis -- 10.2.Results -- 10.2.1.Mutual Antagonism of RB14-C and BY -- 10.2.2.Inhibition of R. solani in Microcosm Studies -- 10.2.3.Biocontrol of R. solani in Pot Experiments -- 10.2.4.Population of R. solani in Soil -- 10.2.5.Number of B. subtilis RB14-C in Soil -- 10.3.Discussion -- References -- 11.1.Mass Production of B. subtilis Culture Broth -- 11.2.Application of B. subtilis to Cherry -- 11.3.Chemical Pesticide Test -- 11.4.Application to Grapes -- 11.5.Advantages of B. subtilis Use.
520 $a "Plant diseases are a serious threat to food production. This unique volume provides the fundamental knowledge and practical use of B.subtilis as a promising biocontrol agent. In order to replace chemical pesticides, one possibility is microbial pesticides using safe microbes. Bacillus subtilis is one of several candidates. Screening of the bacterium, the application of plant tests, clarification of its suppressive mechanism to plant pathogens and engineering aspects of suppressive peptides production are presented here. The author illustrates how B. subtilis is far more advantageous than, for example, Pseudomonas in biocontrol and can be considered as an useful candidate"-- $c Provided by publisher.
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650 0 $a Bacillus subtilis $x Biotechnology. $3 885690
650 0 $a Phytopathogenic microorganisms $x Biological control. $3 192836
776 0 8 $i Online version: $a Shoda, Makoto, $t Biocontrol of plant diseases by bacillus subtilis $b 1. $d Boca Raton : CRC Press, 2019. $z 9780429027635 $w (DLC) 2019030558
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