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Synthesis of a Reversible Crosslinke...

Smith, Kelti A.

Synthesis of a Reversible Crosslinked Hydrogel.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Synthesis of a Reversible Crosslinked Hydrogel.
Author:	Smith, Kelti A.
Published:	Ann Arbor : ProQuest Dissertations & Theses, 2020
Description:	92 p.
Notes:	Source: Masters Abstracts International, Volume: 82-06.
Notes:	Advisor: Shoichet, Molly S.
Contained By:	Masters Abstracts International82-06.
Subject:	Chemical engineering.
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28030172
ISBN:	9798698546719

Synthesis of a Reversible Crosslinked Hydrogel.
Smith, Kelti A.

Synthesis of a Reversible Crosslinked Hydrogel. - Ann Arbor : ProQuest Dissertations & Theses, 2020 - 92 p.

Source: Masters Abstracts International, Volume: 82-06.

Thesis (M.A.S.)--University of Toronto (Canada), 2020.

This item must not be sold to any third party vendors.

Detachment of fragile cell types cultured on two-dimensional (2D) surfaces detrimentally affects cell viability. Accutase treatment of neurons growing in 2D damaged fragile neuronal connections, resulting in cell death and host tissue damage in vivo. Avoiding cell detachment altogether by developing an in vitro culture system may better maintain cell viability; therefore, we present the synthesis of a reversible crosslinked three-dimensional (3D) hydrogel which would provide stability for culture/differentiation when crosslinked and injectability for transplantation following crosslink degradation. A blend of hyaluronan (HA) and methylcellulose (MC), HAMC, was chemically crosslinked with an enzyme-degradable peptide sequence using oxime click-chemistry. Specifically, HA and MC were modified with ketone and aldehyde groups, respectively, and a TEV protease-degradable peptide crosslinker was synthesized and modified with oxyamine groups. The gel components were characterized, and gels were formed that demonstrated good stability, improved injectability upon crosslink degradation, and cytocompatibility with iPSC-derived neural progenitor cells.

ISBN: 9798698546719Subjects--Topical Terms:

206267
Chemical engineering.
Subjects--Index Terms:

Degradable

Synthesis of a Reversible Crosslinked Hydrogel.
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245 1 0 $a Synthesis of a Reversible Crosslinked Hydrogel.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2020
300 $a 92 p.
500 $a Source: Masters Abstracts International, Volume: 82-06.
500 $a Advisor: Shoichet, Molly S.
502 $a Thesis (M.A.S.)--University of Toronto (Canada), 2020.
506 $a This item must not be sold to any third party vendors.
520 $a Detachment of fragile cell types cultured on two-dimensional (2D) surfaces detrimentally affects cell viability. Accutase treatment of neurons growing in 2D damaged fragile neuronal connections, resulting in cell death and host tissue damage in vivo. Avoiding cell detachment altogether by developing an in vitro culture system may better maintain cell viability; therefore, we present the synthesis of a reversible crosslinked three-dimensional (3D) hydrogel which would provide stability for culture/differentiation when crosslinked and injectability for transplantation following crosslink degradation. A blend of hyaluronan (HA) and methylcellulose (MC), HAMC, was chemically crosslinked with an enzyme-degradable peptide sequence using oxime click-chemistry. Specifically, HA and MC were modified with ketone and aldehyde groups, respectively, and a TEV protease-degradable peptide crosslinker was synthesized and modified with oxyamine groups. The gel components were characterized, and gels were formed that demonstrated good stability, improved injectability upon crosslink degradation, and cytocompatibility with iPSC-derived neural progenitor cells.
590 $a School code: 0779.
650 4 $a Chemical engineering. $3 206267
650 4 $a Biomedical engineering. $3 190330
653 $a Degradable
653 $a Dynamic
653 $a Hyaluronan
653 $a Hydrogel
653 $a Methylcellulose
653 $a TEV protease
690 $a 0542
690 $a 0541
710 2 $a University of Toronto (Canada). $b Chemical Engineering & Applied Chemistry. $3 857358
773 0 $t Masters Abstracts International $g 82-06.
790 $a 0779
791 $a M.A.S.
792 $a 2020
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28030172