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Immunologyoverview and laboratory ma...

Sam-Yellowe, Tobili Y.

Immunologyoverview and laboratory manual /

Record Type:	Electronic resources : Monograph/item
Title/Author:	Immunologyby Tobili Y. Sam-Yellowe.
Reminder of title:	overview and laboratory manual /
Author:	Sam-Yellowe, Tobili Y.
Published:	Cham :Springer International Publishing :2021.
Description:	xx, 471 p. :ill., digital ;24 cm.
Contained By:	Springer Nature eBook
Subject:	Immunology.
Online resource:	https://doi.org/10.1007/978-3-030-64686-8
ISBN:	9783030646868$q(electronic bk.)

Immunologyoverview and laboratory manual /
Sam-Yellowe, Tobili Y.

Immunologyoverview and laboratory manual /[electronic resource] :by Tobili Y. Sam-Yellowe. - Cham :Springer International Publishing :2021. - xx, 471 p. :ill., digital ;24 cm.

Part I: Overview of Immunity -- Ch 1: Immunity -- Ch 2: Components of immunity -- Ch 3: Hematopoiesis -- Ch 4: The Immune system: pathogen sensing and detection -- Ch 5: Lymphoid organs -- Ch 6: Innate and Adaptive immunity -- Ch 7: Leukocyte homing, migration and recirculation -- Ch 8: Apoptosis -- Ch 9: Antigens -- Ch 10: B cell development, activation and immunoglobulin structure -- Ch 11: Immunoglobulin and T cell receptor gene rearrangements -- Ch 12: Major histocompatibility complex proteins -- Ch 13: T cell development and T cell receptor structure -- Ch 14: Cytokines -- Ch 15: Lymphocyte signals -- Ch 16: Complement fixation -- Ch 17: Cell mediated immunity -- Ch 18: Hypersensitivities -- Ch 19: Parasite Immunity -- Ch 20: Vaccines -- Ch 21: Transplantation -- Ch 22: Immune regulation and autoimmunity -- Ch 23: Immunodeficiencies -- Ch 24: Cancer -- Ch 25: Practice questions -- Ch 26: Study guide for exam. Part II. Laboratory Manual -- 27. Immunology laboratory policies -- 28. Instructions for notebook entries -- 29. Instructions for writing lab reports -- 30. Lab report topics -- 31. Exercise 1: Using a compound microscope -- 32. Exercise 2: Pipetting and dilution technique, identification of mouse lymphoid organs -- 33. Exercise 3: Blood Cell Preparation, Leukocyte differentiation and ABO Blood Typing -- 34. Exercise 4: Single Suspension of Mouse Spleen cells, Cell Viability Assays, and Identification of specific cells using surface antigens -- 35. Exercise 5: Isolation of mouse peritoneal macrophages: In vitro phagocytosis of bacterial cells -- 36. Exercise 6: Clearance of bacteria from mouse blood by the RES -- 37. Exercise 7: Hemolytic Plaque assay (The Jeme Plaque Assay) -- 38. EXERCISE 8: Rabbit and Mouse Immunizations- Preparation of Polyclonal antibodies and screening assays -- 39. EXERCISE 9: Determination of Antibody Titer: Screening Techniques -- 40. EXERCISE 10: Immunodiffusion and Immunoelectrophoresis -- 41. EXERCISE 11: Protein Affinity Purification of Rabbit IgG -- 42. EXERCISE 12: Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) -- 43. EXERCISE 13: Immunoblotting (Western Blotting) -- 44. Exercise 14: Protein Assay -- 45. Exercise 15: Enzyme-linked Immunosorbent Assay (ELISA) -- 46. EXERCISE 16: Enzyme-Linked Immunospot Assay (ELISPOT) -- 47. EXERCISE 17: Immunofluorescence assay (IFA) -- 48. EXERCISE 18: Flow Cytometry -- 49.EXERCISE 19: Complement Fixation Assays -- 50. Tissue Cell Culture Techniques -- 51. EXERCISE 20: Mitogen Induced Response of Lymphocytes: Detection of Band T cell Lymphocytes Using Immunofluorescence -- 52. Exercise 21: Induction of IL-2 Secretion from Mouse Lymphoma Cells -- 53. EXERCISE 22: Adoptive Transfer of Lymphocytes: Band T cell Cooperation for Antibody Production -- 54. EXERCISE 23: Macrophage Migration Inhibition -- 55. EXERCISE 24: Monoclonal Antibody Preparation -- 56. EXERCISE 25: Immunization of mice with a recombinant protein -- 57. EXERCISE 26: Polarization of T helper cells into TH1 subset -- 58. Exercise 27: Immunoprecipitation -- 59. EXERCISE 28: Induction of apoptosis in T lymphocytes and detection of apoptotic markers -- 60. EXERCISE 29: Cloning and sequencing of cDNAs of mouse variable regions of H and L chains of monoclonal antibodies -- Appendix -- Sample Practice Questions.

A two-in-one text providing teaching lab students with an overview of immunology as well as a lab manual complete with current standard exercises. Section I of this book provides an overview of the immune system and immunity, and includes review questions, problem sets, case studies, inquiry-based questions, and more to provide students with a strong foundation in the field. Section II consists of twenty-two lab exercises focused on key concepts in immunology, such as antibody production, cell separation, cell function, immunoassays, Th1/Th2 cytokine detection, cell and tissue culture methods, and cell and molecular biology techniques. Appendices include safety information, suggested links and readings, and standard discipline processes, protocols, and instructions.

ISBN: 9783030646868$q(electronic bk.)

Standard No.: 10.1007/978-3-030-64686-8doiSubjects--Topical Terms:

189152
Immunology.

LC Class. No.: QR181

Dewey Class. No.: 571.96

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505 0 $a Part I: Overview of Immunity -- Ch 1: Immunity -- Ch 2: Components of immunity -- Ch 3: Hematopoiesis -- Ch 4: The Immune system: pathogen sensing and detection -- Ch 5: Lymphoid organs -- Ch 6: Innate and Adaptive immunity -- Ch 7: Leukocyte homing, migration and recirculation -- Ch 8: Apoptosis -- Ch 9: Antigens -- Ch 10: B cell development, activation and immunoglobulin structure -- Ch 11: Immunoglobulin and T cell receptor gene rearrangements -- Ch 12: Major histocompatibility complex proteins -- Ch 13: T cell development and T cell receptor structure -- Ch 14: Cytokines -- Ch 15: Lymphocyte signals -- Ch 16: Complement fixation -- Ch 17: Cell mediated immunity -- Ch 18: Hypersensitivities -- Ch 19: Parasite Immunity -- Ch 20: Vaccines -- Ch 21: Transplantation -- Ch 22: Immune regulation and autoimmunity -- Ch 23: Immunodeficiencies -- Ch 24: Cancer -- Ch 25: Practice questions -- Ch 26: Study guide for exam. Part II. Laboratory Manual -- 27. Immunology laboratory policies -- 28. Instructions for notebook entries -- 29. Instructions for writing lab reports -- 30. Lab report topics -- 31. Exercise 1: Using a compound microscope -- 32. Exercise 2: Pipetting and dilution technique, identification of mouse lymphoid organs -- 33. Exercise 3: Blood Cell Preparation, Leukocyte differentiation and ABO Blood Typing -- 34. Exercise 4: Single Suspension of Mouse Spleen cells, Cell Viability Assays, and Identification of specific cells using surface antigens -- 35. Exercise 5: Isolation of mouse peritoneal macrophages: In vitro phagocytosis of bacterial cells -- 36. Exercise 6: Clearance of bacteria from mouse blood by the RES -- 37. Exercise 7: Hemolytic Plaque assay (The Jeme Plaque Assay) -- 38. EXERCISE 8: Rabbit and Mouse Immunizations- Preparation of Polyclonal antibodies and screening assays -- 39. EXERCISE 9: Determination of Antibody Titer: Screening Techniques -- 40. EXERCISE 10: Immunodiffusion and Immunoelectrophoresis -- 41. EXERCISE 11: Protein Affinity Purification of Rabbit IgG -- 42. EXERCISE 12: Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) -- 43. EXERCISE 13: Immunoblotting (Western Blotting) -- 44. Exercise 14: Protein Assay -- 45. Exercise 15: Enzyme-linked Immunosorbent Assay (ELISA) -- 46. EXERCISE 16: Enzyme-Linked Immunospot Assay (ELISPOT) -- 47. EXERCISE 17: Immunofluorescence assay (IFA) -- 48. EXERCISE 18: Flow Cytometry -- 49.EXERCISE 19: Complement Fixation Assays -- 50. Tissue Cell Culture Techniques -- 51. EXERCISE 20: Mitogen Induced Response of Lymphocytes: Detection of Band T cell Lymphocytes Using Immunofluorescence -- 52. Exercise 21: Induction of IL-2 Secretion from Mouse Lymphoma Cells -- 53. EXERCISE 22: Adoptive Transfer of Lymphocytes: Band T cell Cooperation for Antibody Production -- 54. EXERCISE 23: Macrophage Migration Inhibition -- 55. EXERCISE 24: Monoclonal Antibody Preparation -- 56. EXERCISE 25: Immunization of mice with a recombinant protein -- 57. EXERCISE 26: Polarization of T helper cells into TH1 subset -- 58. Exercise 27: Immunoprecipitation -- 59. EXERCISE 28: Induction of apoptosis in T lymphocytes and detection of apoptotic markers -- 60. EXERCISE 29: Cloning and sequencing of cDNAs of mouse variable regions of H and L chains of monoclonal antibodies -- Appendix -- Sample Practice Questions.
520 $a A two-in-one text providing teaching lab students with an overview of immunology as well as a lab manual complete with current standard exercises. Section I of this book provides an overview of the immune system and immunity, and includes review questions, problem sets, case studies, inquiry-based questions, and more to provide students with a strong foundation in the field. Section II consists of twenty-two lab exercises focused on key concepts in immunology, such as antibody production, cell separation, cell function, immunoassays, Th1/Th2 cytokine detection, cell and tissue culture methods, and cell and molecular biology techniques. Appendices include safety information, suggested links and readings, and standard discipline processes, protocols, and instructions.
650 0 $a Immunology. $3 189152
650 0 $a Immunology $3 253093
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856 4 0 $u https://doi.org/10.1007/978-3-030-64686-8
950 $a Biomedical and Life Sciences (SpringerNature-11642)