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製備具標靶葉酸受體診斷及治療的多功能pluronic修飾四氧化三鐵 = ...

國立高雄大學生物科技研究所

製備具標靶葉酸受體診斷及治療的多功能pluronic修飾四氧化三鐵 = Folate-Mediated Pluronic Modified Fe3O4 for Combined Diagnosis and Therapy

Record Type:	Language materials, printed : monographic
Paralel Title:	Folate-Mediated Pluronic Modified Fe3O4 for Combined Diagnosis and Therapy
Author:	林嘉鈞,
Secondary Intellectual Responsibility:	國立高雄大學
Place of Publication:	[高雄市]
Published:	撰者;
Year of Publication:	2009[民98]
Description:	[8], 67面圖，表 : 30公分;
Subject:	Pluronic F127
Subject:	Diagnosis
Online resource:	http://handle.ncl.edu.tw/11296/ndltd/66533734059769910024
Notes:	指導教授：陳振興
Notes:	參考書目：面66-67
Summary:	核磁共振造影最大的貢獻在於能在不同的組織和不同的環境下，呈現出有差異的顯影效果，因此被用來診斷組織的病變與否。本研究中，利用Pluronic®高分子F127(PF127) 的PPO疏水端攜帶疏水性藥物，與水溶性的四氧化三鐵(PAAIO)反應，並在PF127親水端接枝上葉酸(folic acid, FA)做為標靶癌細胞的配體分子，形成一個具備診斷、治療、標靶的多功能磁性微胞(FA-PF127-PAAIO)。並以無葉酸標的的磁性微胞(PF127-PAAIO)作為對照組。使用紅外線光譜儀觀測磁性微胞酯鍵的吸收。紫外線吸收光譜儀觀察到葉酸的特殊吸收訊號。修飾後的磁性微胞從TEM、DLS觀察粒徑大小，在介面電位也有明顯的變化。AAS組成比例分析磁性微胞的含鐵量。超導量子干涉儀分析上，由磁滯曲線測得超順磁性以及飽和磁化率。細胞實驗裡，我們利用流式細胞儀與體外磁振造影實驗分析含葉酸的FA-PF127-PAAIO標的癌細胞的表現，初步的結果說明FA-PF127-PAAIO有標靶葉酸受體的能力。 Magnetic resonance (MR) tracking of superparamagnetic iron oxide labeled cells is a relatively new technique to non-invasively determine the cancer cells.In this study, Pluronic® F127 (PF127), which is capable of encapsulating hydrophobic drug due to its PPO hydrophobic segments, was proceeded a condensation reaction with water-soluble polyacrylic acid bound iron oxide (PAAIO). A tumor-specific targeting ligand, folic acid (FA), was also grafted onto PF127-PAAIO to give FA-PF127-PAAIO magnetic micelles. A multifunctional FA-PF127-PAAIO can be used as combined diagnosis and therapy purposes specifically targeting to cancer cells. PF127-PAAIO magnetic micelles without folic acid were used as a control group. According to IR and UV absorption spectra, we ensured the successful preparation of the magnetic micelles with polymeric compartments for carrying a drug. The hysteresis curves of SQUID magnetometer analysis also showed that these magnetic micelles were superparamagnetic and a saturation magnetization was obtained in FA-PF127-PAAIO and PF127-PAAIO.Internalization of FA-PF127-PAAIO in targeted cells (KB, A549 cells) was observed by flow-cytometric analysis and in vitro MR imaging. These preliminary results demonstrated that FA-PF127-PAAIO have the ability to target folate receptor.

製備具標靶葉酸受體診斷及治療的多功能pluronic修飾四氧化三鐵 = Folate-Mediated Pluronic Modified Fe3O4 for Combined Diagnosis and Therapy
林, 嘉鈞

製備具標靶葉酸受體診斷及治療的多功能pluronic修飾四氧化三鐵 = Folate-Mediated Pluronic Modified Fe3O4 for Combined Diagnosis and Therapy / 林嘉鈞撰 - [高雄市] : 撰者, 2009[民98]. - [8], 67面 ; 圖，表 ; 30公分.
指導教授：陳振興參考書目：面66-67.
Pluronic F127Diagnosis

製備具標靶葉酸受體診斷及治療的多功能pluronic修飾四氧化三鐵 = Folate-Mediated Pluronic Modified Fe3O4 for Combined Diagnosis and Therapy
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