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Biochemical and cellular aspects of antigen presentation by human CD1d.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Biochemical and cellular aspects of antigen presentation by human CD1d.
Author:	Kang, Suk-Jo.
Description:	131 p.
Notes:	Director: Peter Cresswell.
Notes:	Source: Dissertation Abstracts International, Volume: 64-03, Section: B, page: 1166.
Contained By:	Dissertation Abstracts International64-03B.
Subject:	Health Sciences, Immunology.
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3084316
ISBN:	0496321234

Biochemical and cellular aspects of antigen presentation by human CD1d.
Kang, Suk-Jo.

Biochemical and cellular aspects of antigen presentation by human CD1d. [electronic resource] - 131 p.

Director: Peter Cresswell.

Thesis (Ph.D.)--Yale University, 2003.

A subset of human CD1d molecules were found to be associated with MHC class II molecules, both on the cell surface and in the late endosomal/lysosomal compartments where class II molecules transiently accumulate during transport. The interaction is initiated in the ER with class II-invariant chain complexes and appears to be maintained throughout the class II trafficking pathway. A truncated form of CD1d which lacks its cytoplasmic domain and therefore the YXXZ internalization motif fails to be transported to late endosomal/lysosomal compartments in a mutant B cell line which lacks MHC class II expression. However, the same CD1d deletion mutant is targeted to lysosomal compartments in B cell lines expressing class II molecules and HeLa cells expressing class II molecules and invariant chain by transfection. The deletion mutant was also found in lysosomal compartments in HeLa cells expressing only the p33 form of the invariant chain. These data suggest that the intracellular trafficking pathway of CD1d may be altered by class II molecules and invariant chain induced during inflammation.

ISBN: 0496321234Subjects--Topical Terms:

226966
Health Sciences, Immunology.

Biochemical and cellular aspects of antigen presentation by human CD1d.
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100 0 $a Kang, Suk-Jo. $3 226965
245 1 0 $a Biochemical and cellular aspects of antigen presentation by human CD1d. $h [electronic resource]
300 $a 131 p.
500 $a Director: Peter Cresswell.
500 $a Source: Dissertation Abstracts International, Volume: 64-03, Section: B, page: 1166.
502 $a Thesis (Ph.D.)--Yale University, 2003.
520 # $a A subset of human CD1d molecules were found to be associated with MHC class II molecules, both on the cell surface and in the late endosomal/lysosomal compartments where class II molecules transiently accumulate during transport. The interaction is initiated in the ER with class II-invariant chain complexes and appears to be maintained throughout the class II trafficking pathway. A truncated form of CD1d which lacks its cytoplasmic domain and therefore the YXXZ internalization motif fails to be transported to late endosomal/lysosomal compartments in a mutant B cell line which lacks MHC class II expression. However, the same CD1d deletion mutant is targeted to lysosomal compartments in B cell lines expressing class II molecules and HeLa cells expressing class II molecules and invariant chain by transfection. The deletion mutant was also found in lysosomal compartments in HeLa cells expressing only the p33 form of the invariant chain. These data suggest that the intracellular trafficking pathway of CD1d may be altered by class II molecules and invariant chain induced during inflammation.
520 # $a By using soluble secreted and ER-retained CD1d molecules, phosphatidylinositol was identified as a natural ligand that CD1d binds in the ER in vivo .
520 # $a CD1d associates in the ER with the chaperones calnexin and calreticulin and with the thiol oxidoreductase ERp57 in a manner dependent on glucose trimming of its N-linked glycans. Complete disulfide bond formation in the CD1d heavy chain was substantially impaired if the chaperone interactions were blocked by glucosidase inhibitors. The formation of at least one of the disulfide bonds in the CD1d heavy chain is coupled to its glucose-trimming-dependent association with ERp57, calnexin and calreticulin.
520 # $a Members of the CD1 family of membrane glycoproteins can present antigenic lipids to T lymphocytes. Like MHC class I molecules, they form a heterodimeric complex of a heavy chain and beta2-microglobulin (beta2m) in the ER. Binding of lipid antigens, however, takes place in endosomal compartments, similar to class II molecules, and on the plasma membrane. The studies presented here try to understand the mechanisms governing the assembly of human CD1d in the endoplasmic reticulum (ER) and its intracellular trafficking to lysosomes, as well as identifying the endogenous ligands for CD1d.
590 $a School code: 0265.
650 # 0 $a Health Sciences, Immunology. $3 226966
650 # 0 $a Chemistry, Biochemistry. $3 226900
650 # 0 $a Biology, Cell. $3 226967
710 0 # $a Yale University. $3 212430
773 0 # $g 64-03B. $t Dissertation Abstracts International
790 $a 0265
790 1 0 $a Cresswell, Peter, $e advisor
791 $a Ph.D.
792 $a 2003
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