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In situ formation and characterizati...

Munro, Jeffrey Christian.

In situ formation and characterization of polymer-supported lipid bilayers.

Record Type:	Electronic resources : Monograph/item
Title/Author:	In situ formation and characterization of polymer-supported lipid bilayers.
Author:	Munro, Jeffrey Christian.
Description:	252 p.
Notes:	Adviser: Curtis W. Frank.
Notes:	Source: Dissertation Abstracts International, Volume: 65-09, Section: B, page: 4710.
Contained By:	Dissertation Abstracts International65-09B.
Subject:	Engineering, Chemical.
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3145564
ISBN:	0496045261

In situ formation and characterization of polymer-supported lipid bilayers.
Munro, Jeffrey Christian.

In situ formation and characterization of polymer-supported lipid bilayers. - 252 p.

Adviser: Curtis W. Frank.

Thesis (Ph.D.)--Stanford University, 2004.

Finally, mobile, tethered lipid bilayers were formed on the adsorbed PEG films via a two-step adsorption process. First, lipids were adsorbed onto the PEG support from a hexane solution. Second, vesicles were adsorbed and fused on the surface to create a bilayer in an aqueous environment. Fluorescence recovery after photobleaching experiments show that this process results in mobile bilayers with diffusion constants on the order of 2 mum 2/s.

ISBN: 0496045261Subjects--Topical Terms:

226989
Engineering, Chemical.

In situ formation and characterization of polymer-supported lipid bilayers.
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245 1 0 $a In situ formation and characterization of polymer-supported lipid bilayers.
300 $a 252 p.
500 $a Adviser: Curtis W. Frank.
500 $a Source: Dissertation Abstracts International, Volume: 65-09, Section: B, page: 4710.
502 $a Thesis (Ph.D.)--Stanford University, 2004.
520 # $a Finally, mobile, tethered lipid bilayers were formed on the adsorbed PEG films via a two-step adsorption process. First, lipids were adsorbed onto the PEG support from a hexane solution. Second, vesicles were adsorbed and fused on the surface to create a bilayer in an aqueous environment. Fluorescence recovery after photobleaching experiments show that this process results in mobile bilayers with diffusion constants on the order of 2 mum 2/s.
520 # $a First, in order to better understand the properties of the polymer cushion, an extensive study of the viscoelastic properties of thin polyacrylamide (PA) films was performed with a quartz crystal microbalance with dissipation (QCM-D). Using a continuum mechanics viscoelastic film model, we extract film property parameters from frequency and dissipation change measurements for multiple harmonics.
520 # $a Second, we have studied the adsorption behavior and viscoelastic properties of two lipopolymers: a PA-based random copolymer and an end-functionalized poly (ethylene glycol) (PEG). Both lipopolymers are functionalized with lipids for anchoring into a bilayer and disulfides for covalent attachment to gold or silver surfaces. The adsorption of both lipopolymers to gold surfaces has been studied using a variety of surface-sensitive characterization techniques. Our experiments indicate that the PEG lipopolymer has several favorable characteristics compared to the PA-based lipopolymer, including thicker polymer cushions, more complete chemisorption to the surface, and better control over the tethered lipid density. Additional experiments indicate that the position of the lipid end groups can be controlled by adsorbing the PEG lipopolymer from various solvents. Finally, the surface density of tethered lipid groups can be controlled by co-adsorbing the PEG lipopolymer with an analogous methoxy-terminated PEG molecule.
520 # $a Tethered lipid bilayer membranes provide a model for cellular systems and have potential applications in areas such as biosensors. Inclusion of a polymer cushion between a lipid bilayer membrane and solid surface has been suggested as a means to provide a soft, deformable layer that will allow for transmembrane protein insertion and mobility. In this work, we present methods for the self-assembly of multifunctional polymers that provide a substantial hydrophilic cushion to create tethered lipid bilayers in situ.
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790 1 0 $a Frank, Curtis W., $e advisor
791 $a Ph.D.
792 $a 2004
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