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DNA Aptamer Development For Detectio...

Sanchez, Pablo Enrique.

DNA Aptamer Development For Detection Of Atrazine And Protective Antigen Toxin Using Fluorescence Polarization.

Record Type:	Electronic resources : Monograph/item
Title/Author:	DNA Aptamer Development For Detection Of Atrazine And Protective Antigen Toxin Using Fluorescence Polarization.
Author:	Sanchez, Pablo Enrique.
Description:	99 p.
Notes:	Source: Dissertation Abstracts International, Volume: 73-08(E), Section: B.
Notes:	Adviser: Ashok Mulchandani.
Contained By:	Dissertation Abstracts International73-08B(E).
Subject:	Health Sciences, Toxicology.
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3503295
ISBN:	9781267268334

DNA Aptamer Development For Detection Of Atrazine And Protective Antigen Toxin Using Fluorescence Polarization.
Sanchez, Pablo Enrique.

DNA Aptamer Development For Detection Of Atrazine And Protective Antigen Toxin Using Fluorescence Polarization. - 99 p.

Source: Dissertation Abstracts International, Volume: 73-08(E), Section: B.

Thesis (Ph.D.)--University of California, Riverside, 2012.

Aptamers are becoming a viable alternative to antibodies as bio-recognition elements in analytical, diagnostic and therapeutic applications. In this research two aptamers were developed using capillary electrophoresis systematic evolution of ligands by exponential enrichment (CE-SELEX). These aptamers were selected from and artificial library of single stranded deoxyribonucleic acid (ssDNA) with 40 randomized nucleotides flanked by two priming sites with a diversity of 1015 different sequences.

ISBN: 9781267268334Subjects--Topical Terms:

227089
Health Sciences, Toxicology.

DNA Aptamer Development For Detection Of Atrazine And Protective Antigen Toxin Using Fluorescence Polarization.
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100 1 $a Sanchez, Pablo Enrique. $3 603230
245 1 0 $a DNA Aptamer Development For Detection Of Atrazine And Protective Antigen Toxin Using Fluorescence Polarization.
300 $a 99 p.
500 $a Source: Dissertation Abstracts International, Volume: 73-08(E), Section: B.
500 $a Adviser: Ashok Mulchandani.
502 $a Thesis (Ph.D.)--University of California, Riverside, 2012.
520 $a Aptamers are becoming a viable alternative to antibodies as bio-recognition elements in analytical, diagnostic and therapeutic applications. In this research two aptamers were developed using capillary electrophoresis systematic evolution of ligands by exponential enrichment (CE-SELEX). These aptamers were selected from and artificial library of single stranded deoxyribonucleic acid (ssDNA) with 40 randomized nucleotides flanked by two priming sites with a diversity of 1015 different sequences.
520 $a The first target employed in this study was the extensively used herbicide, atrazine, for which and aptamer with dissociation constant of 890 nM was selected.
520 $a The second target was Protective antigen toxin from the gram positive spore forming bacteria Bacillus anthracis for which an aptamer with a dissociation constant of 112 nM was selected from the library.
520 $a These two aptamers were used to construct a bioassay based on fluorescence polarization, for the successful detection of their respective targets in spiked samples under different conditions.
590 $a School code: 0032.
650 4 $a Health Sciences, Toxicology. $3 227089
650 4 $a Biology, Microbiology. $3 226920
650 4 $a Engineering, Chemical. $3 226989
650 4 $a Engineering, Environmental. $3 212478
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710 2 $a University of California, Riverside. $b Microbiology. $3 603231
773 0 $t Dissertation Abstracts International $g 73-08B(E).
790 1 0 $a Mulchandani, Ashok, $e advisor
790 1 0 $a Walker, Sharon $e committee member
790 1 0 $a Ge, Xin $e committee member
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791 $a Ph.D.
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