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阿拉比卡咖啡試管內播種及癒傷組織之誘導 = In vitro germi...

國立高雄大學生命科學系碩士班

阿拉比卡咖啡試管內播種及癒傷組織之誘導 = In vitro germination and callus induction of Coffee arabica L.

Record Type:	Language materials, printed : monographic
Paralel Title:	In vitro germination and callus induction of Coffee arabica L.
Author:	林孟澤,
Secondary Intellectual Responsibility:	國立高雄大學
Place of Publication:	[高雄市]
Published:	撰者;
Year of Publication:	2014[民103]
Description:	54面圖，表 : 30公分;
Subject:	癒傷組織
Subject:	cytokinin
Online resource:	http://handle.ncl.edu.tw/11296/ndltd/30732539050831175137
Notes:	參考書目：面39-47
Notes:	103年12月16日公開
Summary:	本論文建立阿拉比卡咖啡癒傷組織之誘導系統，首先測試種子無菌萌芽之培養條件，並藉以獲取足量之實生苗供後續試驗。切取阿拉比卡咖啡實生苗的根及葉為培植體，置於含不同種類及濃度植物生長調節劑的Murashige and Skoog（MS）培養基，以全暗培養進行癒傷組織之誘導，將誘導所得之癒傷組織進行繼代增殖，及試管內形態發生試驗。種子萌芽試驗結果顯示，以濃度0.5%之次氯酸鈉消毒10分鐘，可獲得最高萌芽比率（63.16%）。切取萌芽十二週實生苗之根及葉做為培植體，以不同植物生長調節劑（plant growth regulators，PGRs），包括植物生長素α-naphthaleneacetic acid （NAA）或2,4-dichlorophenoxyacetic acid（2,4-D）與細胞分裂素thidiazuron（TDZ）或N6-benzyladenine（BA），搭配進行癒傷組織誘導試驗。癒傷組織誘導試驗結果顯示，根及葉培植體在不含PGR的控制組中，無法誘導出癒傷組織。然而，其餘各試驗組皆可成功誘導出癒傷組織，共獲得二十八個品系癒傷組織，這些癒傷組織可穩定生長，並以相同條件的培養基持續進行繼代，經持續觀察得知，各品系癒傷組織之外觀、顏色、形態及結構有所差異。癒傷組織增殖率試驗及形態分析結果顯示，可將癒傷組織分為四種：第一種為結構緊密、觸感堅硬之黃色癒傷組織，生長速度最快；第二種為蓬鬆顆粒狀且觸感略硬之淡黃色且透白的癒傷組織，生長速度次之；第三種為結構鬆散、觸感軟綿之白色半透明狀癒傷組織，生長速度稍慢；第四種為形態軟爛之紅褐色癒傷組織，生長速度緩慢。本論文成功建立了阿拉比卡咖啡的癒傷組織誘導及增殖系統，建議未來可以選擇本論文所篩選出之生長快速、質地緊密及外觀顏色呈淡黃色或白色之癒傷組織，進一步建立完整的植株再生體系。 The thesis attempts to establish a protocol for callus induction of Coffee arabica L. Firstly, the seeds were sown in in vitro conditions and seedlings obtained were used as donor plants for the subsequent tests. The explants were taken from these donor plants and placed on a modified Murashige and Skoog (MS) medium supplemented with combinations of plant growth regulators (PGRs) in the dark. The calli were proliferated more on the same medium, and their capacities of in vitro morphogenesis were subsequently evaluated. In the seeds germination experiment, 10 min sterilization time with 0.5% sodium hypochlorite gave the highest germination rate (63.16%). The root and leaf explants were taken from 12-week-old seedings and placed on a modified MS medium supplemented with combinations of auxins, (α-naphthaleneacetic acid : NAA or 2,4-dichlorophenoxyacetic acid : 2,4-D) and cytokinins (thidiazuron : TDZ or N6-benzyladenine : BA). In the control treatment, no response was found at all the explants. In contrast, 28 callus lines were obtained from other treatments. These calli could be subcultured on same medium and proliferated more with viable growth. Based on the proliferation rate and their morphology, the callus were classified into 4 types : 1) Yellowish compact callus with a highest proliferation rate; 2) Pale yellowish granular callus with high proliferation rate; 3) Translucent to whitish and friable callus with low proliferation rate; 4) Red to brown callus with lowest proliferation rate. Type 1 callus lines were suggested for inducing in vitro morphogenesis and subsequent plant regeneration.

阿拉比卡咖啡試管內播種及癒傷組織之誘導 = In vitro germination and callus induction of Coffee arabica L.
林, 孟澤

阿拉比卡咖啡試管內播種及癒傷組織之誘導 = In vitro germination and callus induction of Coffee arabica L. / 林孟澤撰 - [高雄市] : 撰者, 2014[民103]. - 54面 ; 圖，表 ; 30公分.
參考書目：面39-47103年12月16日公開.
癒傷組織cytokinin

阿拉比卡咖啡試管內播種及癒傷組織之誘導 = In vitro germination and callus induction of Coffee arabica L.
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330 $a 本論文建立阿拉比卡咖啡癒傷組織之誘導系統，首先測試種子無菌萌芽之培養條件，並藉以獲取足量之實生苗供後續試驗。切取阿拉比卡咖啡實生苗的根及葉為培植體，置於含不同種類及濃度植物生長調節劑的Murashige and Skoog（MS）培養基，以全暗培養進行癒傷組織之誘導，將誘導所得之癒傷組織進行繼代增殖，及試管內形態發生試驗。種子萌芽試驗結果顯示，以濃度0.5%之次氯酸鈉消毒10分鐘，可獲得最高萌芽比率（63.16%）。切取萌芽十二週實生苗之根及葉做為培植體，以不同植物生長調節劑（plant growth regulators，PGRs），包括植物生長素α-naphthaleneacetic acid （NAA）或2,4-dichlorophenoxyacetic acid（2,4-D）與細胞分裂素thidiazuron（TDZ）或N6-benzyladenine（BA），搭配進行癒傷組織誘導試驗。癒傷組織誘導試驗結果顯示，根及葉培植體在不含PGR的控制組中，無法誘導出癒傷組織。然而，其餘各試驗組皆可成功誘導出癒傷組織，共獲得二十八個品系癒傷組織，這些癒傷組織可穩定生長，並以相同條件的培養基持續進行繼代，經持續觀察得知，各品系癒傷組織之外觀、顏色、形態及結構有所差異。癒傷組織增殖率試驗及形態分析結果顯示，可將癒傷組織分為四種：第一種為結構緊密、觸感堅硬之黃色癒傷組織，生長速度最快；第二種為蓬鬆顆粒狀且觸感略硬之淡黃色且透白的癒傷組織，生長速度次之；第三種為結構鬆散、觸感軟綿之白色半透明狀癒傷組織，生長速度稍慢；第四種為形態軟爛之紅褐色癒傷組織，生長速度緩慢。本論文成功建立了阿拉比卡咖啡的癒傷組織誘導及增殖系統，建議未來可以選擇本論文所篩選出之生長快速、質地緊密及外觀顏色呈淡黃色或白色之癒傷組織，進一步建立完整的植株再生體系。 The thesis attempts to establish a protocol for callus induction of Coffee arabica L. Firstly, the seeds were sown in in vitro conditions and seedlings obtained were used as donor plants for the subsequent tests. The explants were taken from these donor plants and placed on a modified Murashige and Skoog (MS) medium supplemented with combinations of plant growth regulators (PGRs) in the dark. The calli were proliferated more on the same medium, and their capacities of in vitro morphogenesis were subsequently evaluated. In the seeds germination experiment, 10 min sterilization time with 0.5% sodium hypochlorite gave the highest germination rate (63.16%). The root and leaf explants were taken from 12-week-old seedings and placed on a modified MS medium supplemented with combinations of auxins, (α-naphthaleneacetic acid : NAA or 2,4-dichlorophenoxyacetic acid : 2,4-D) and cytokinins (thidiazuron : TDZ or N6-benzyladenine : BA). In the control treatment, no response was found at all the explants. In contrast, 28 callus lines were obtained from other treatments. These calli could be subcultured on same medium and proliferated more with viable growth. Based on the proliferation rate and their morphology, the callus were classified into 4 types : 1) Yellowish compact callus with a highest proliferation rate; 2) Pale yellowish granular callus with high proliferation rate; 3) Translucent to whitish and friable callus with low proliferation rate; 4) Red to brown callus with lowest proliferation rate. Type 1 callus lines were suggested for inducing in vitro morphogenesis and subsequent plant regeneration.
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