國立高雄大學圖資館 |

Language: English

Back

Applications of aminoacylation riboz...

SpringerLink (Online service)

Applications of aminoacylation ribozymes that recognize the 3'-end of tRNA

Record Type:	Electronic resources : Monograph/item
Title/Author:	Applications of aminoacylation ribozymes that recognize the 3'-end of tRNAby Naohiro Terasaka.
Author:	Terasaka, Naohiro.
Published:	Tokyo :Springer Japan :2017.
Description:	xii, 89 p. :ill., digital ;24 cm.
Contained By:	Springer eBooks
Subject:	RNA.
Online resource:	http://dx.doi.org/10.1007/978-4-431-56517-8
ISBN:	9784431565178$q(electronic bk.)

Applications of aminoacylation ribozymes that recognize the 3'-end of tRNA
Terasaka, Naohiro.

Applications of aminoacylation ribozymes that recognize the 3'-end of tRNA[electronic resource] /by Naohiro Terasaka. - Tokyo :Springer Japan :2017. - xii, 89 p. :ill., digital ;24 cm. - Springer theses,2190-5053. - Springer theses..

General introduction -- Discovery of human microRNA precursor binding to folic acid by small RNA transcriptomic SELEX -- Orthogonal ribosome-tRNAs pair by engineering of peptidyl transferase center -- General conclusion.

In this thesis, applications of aminoacylation ribozymes named flexizymes are described. Flexizymes have the following unique characteristics: (i) substrate RNA is recognized by two consecutive base pairs between the 3'-end of substrate RNA and the 3'-end of the flexizyme; (ii) these base pairs can be substituted with other base pairs; and (iii) various activated amino acids can be used as substrates including both canonical and noncanonical amino acids. This flexible aminoacylation of RNAs by flexizymes was used to label endogenous tRNAs to be removed, and in vitro selection using the tRNA-depleted library enabled the discovery of the novel interaction between the microRNA precursor and metabolites. Flexizymes are also used to prepare various aminoacyl-tRNAs bearing mutations at the 3'-end to engineer the translation machinery and to develop the orthogonal translation machinery. The first part of the research demonstrated that SELEX is appropriate for discovering the interaction between small RNA and ligands, and suggested that more RNA motif binding to small molecules exists in small RNAs. The second part opened a door to new opportunities for in vitro synthetic biology involving the engineering of the genetic codes and translation machineries. This research also indicated the great potential of aminoacylation by flexizymes to be applied in various fields of RNA research, which is beneficial for RNA researchers.

ISBN: 9784431565178$q(electronic bk.)

Standard No.: 10.1007/978-4-431-56517-8doiSubjects--Topical Terms:

216963
RNA.

LC Class. No.: QP619.A45

Dewey Class. No.: 572.886

Applications of aminoacylation ribozymes that recognize the 3'-end of tRNA
LDR:02702nmm a2200337 a 4500 001 508863
003 DE-He213
005 20170309113605.0
006 m d
007 cr nn 008maaau
008 171121s2017 ja s 0 eng d
020 $a 9784431565178$q(electronic bk.)
020 $a 9784431565154$q(paper)
024 7 $a 10.1007/978-4-431-56517-8 $2 doi
035 $a 978-4-431-56517-8
040 $a GP $c GP
041 0 $a eng
050 4 $a QP619.A45
072 7 $a PSB $2 bicssc
072 7 $a SCI013040 $2 bisacsh
072 7 $a SCI007000 $2 bisacsh
082 0 4 $a 572.886 $2 23
090 $a QP619.A45 $b T315 2017
100 1 $a Terasaka, Naohiro. $3 775291
245 1 0 $a Applications of aminoacylation ribozymes that recognize the 3'-end of tRNA $h [electronic resource] / $c by Naohiro Terasaka.
260 $a Tokyo : $b Springer Japan : $b Imprint: Springer, $c 2017.
300 $a xii, 89 p. : $b ill., digital ; $c 24 cm.
490 1 $a Springer theses, $x 2190-5053
505 0 $a General introduction -- Discovery of human microRNA precursor binding to folic acid by small RNA transcriptomic SELEX -- Orthogonal ribosome-tRNAs pair by engineering of peptidyl transferase center -- General conclusion.
520 $a In this thesis, applications of aminoacylation ribozymes named flexizymes are described. Flexizymes have the following unique characteristics: (i) substrate RNA is recognized by two consecutive base pairs between the 3'-end of substrate RNA and the 3'-end of the flexizyme; (ii) these base pairs can be substituted with other base pairs; and (iii) various activated amino acids can be used as substrates including both canonical and noncanonical amino acids. This flexible aminoacylation of RNAs by flexizymes was used to label endogenous tRNAs to be removed, and in vitro selection using the tRNA-depleted library enabled the discovery of the novel interaction between the microRNA precursor and metabolites. Flexizymes are also used to prepare various aminoacyl-tRNAs bearing mutations at the 3'-end to engineer the translation machinery and to develop the orthogonal translation machinery. The first part of the research demonstrated that SELEX is appropriate for discovering the interaction between small RNA and ligands, and suggested that more RNA motif binding to small molecules exists in small RNAs. The second part opened a door to new opportunities for in vitro synthetic biology involving the engineering of the genetic codes and translation machineries. This research also indicated the great potential of aminoacylation by flexizymes to be applied in various fields of RNA research, which is beneficial for RNA researchers.
650 0 $a RNA. $3 216963
650 0 $a Aminoacyl-tRNA synthetases. $3 680238
650 1 4 $a Chemistry. $3 188628
650 2 4 $a Bioorganic Chemistry. $3 275016
650 2 4 $a Catalysis. $3 188259
650 2 4 $a Nucleic Acid Chemistry. $3 376665
650 2 4 $a Biotechnology. $3 188829
710 2 $a SpringerLink (Online service) $3 273601
773 0 $t Springer eBooks
830 0 $a Springer theses. $3 557607
856 4 0 $u http://dx.doi.org/10.1007/978-4-431-56517-8
950 $a Chemistry and Materials Science (Springer-11644)