國立高雄大學圖資館 |

Language: English

Back

Enzyme-linked immunosorbent assay (E...

Hosseini, Samira.

Enzyme-linked immunosorbent assay (ELISA)from A to Z /

Record Type:	Electronic resources : Monograph/item
Title/Author:	Enzyme-linked immunosorbent assay (ELISA)by Samira Hosseini ... [et al.].
Reminder of title:	from A to Z /
other author:	Hosseini, Samira.
Published:	Singapore :Springer Singapore :2018.
Description:	xi, 115 p. :ill., digital ;24 cm.
Contained By:	Springer eBooks
Subject:	Enzyme-linked immunosorbent assay.
Online resource:	http://dx.doi.org/10.1007/978-981-10-6766-2
ISBN:	9789811067662$q(electronic bk.)

Enzyme-linked immunosorbent assay (ELISA)from A to Z /
Enzyme-linked immunosorbent assay (ELISA)from A to Z /[electronic resource] :by Samira Hosseini ... [et al.]. - Singapore :Springer Singapore :2018. - xi, 115 p. :ill., digital ;24 cm. - SpringerBriefs in applied sciences and technology,2191-530X. - SpringerBriefs in applied sciences and technology..

1.Fundamentals of ELISA: from the evolution of the immunoassays until invention of ELISA -- 2.General overview on applications of ELISA -- 3.Step by step with ELISA: mechanism of operation, crucial elements, different protocols, and insights on immobilization and detection of various biomolecular entities -- 4. Evaluation of the detection results obtained from ELISA -- 5. Advantages, disadvantages and modifications of conventional ELISA -- 6.Common questions and answers about ELISA.

This book offers comprehensive information on all aspects of ELISA, starting with the fundamentals of the immune system. It also reviews the history of analytical assays prior to the advent of ELISA (enzyme-linked immunosorbent assay) and addresses the materials of choice for the fabrication of the platforms, possible biomolecular interactions, different protocols, and evaluation parameters. The book guides readers through the respective steps of the analytical assay, while also familiarizing them with the possible sources of error in the assay. It offers detailed insights into the immobilization techniques used for protein attachment, as well as methods for evaluating the assay and calculating the key parameters, such as sensitivity, specificity, accuracy and limit of detection. In addition, the book explores the advantages and shortcomings of the conventional ELISA, as well as various approaches to improving its performance. In this regard, merging and integrating other technologies with widely known ELISAs have opened new avenues for the advancement of this immunoassay. Accordingly, the book provides cutting-edge information on integrated platforms such as ELISpot, plasmonic ELISAs, sphere-/bead-based ELISAs, paper-/fiber-based ELISAs and ELISA in micro-devices.

ISBN: 9789811067662$q(electronic bk.)

Standard No.: 10.1007/978-981-10-6766-2doiSubjects--Topical Terms:

197413
Enzyme-linked immunosorbent assay.

LC Class. No.: QP519.9.E48

Dewey Class. No.: 610.28

Enzyme-linked immunosorbent assay (ELISA)from A to Z /
LDR:02796nmm a2200325 a 4500 001 530007
003 DE-He213
005 20180807170016.0
006 m d
007 cr nn 008maaau
008 181107s2018 si s 0 eng d
020 $a 9789811067662$q(electronic bk.)
020 $a 9789811067655$q(paper)
024 7 $a 10.1007/978-981-10-6766-2 $2 doi
035 $a 978-981-10-6766-2
040 $a GP $c GP
041 0 $a eng
050 4 $a QP519.9.E48
072 7 $a MQW $2 bicssc
072 7 $a TEC009000 $2 bisacsh
082 0 4 $a 610.28 $2 23
090 $a QP519.9.E48 $b E61 2018
245 0 0 $a Enzyme-linked immunosorbent assay (ELISA) $h [electronic resource] : $b from A to Z / $c by Samira Hosseini ... [et al.].
260 $a Singapore : $b Springer Singapore : $b Imprint: Springer, $c 2018.
300 $a xi, 115 p. : $b ill., digital ; $c 24 cm.
490 1 $a SpringerBriefs in applied sciences and technology, $x 2191-530X
505 0 $a 1.Fundamentals of ELISA: from the evolution of the immunoassays until invention of ELISA -- 2.General overview on applications of ELISA -- 3.Step by step with ELISA: mechanism of operation, crucial elements, different protocols, and insights on immobilization and detection of various biomolecular entities -- 4. Evaluation of the detection results obtained from ELISA -- 5. Advantages, disadvantages and modifications of conventional ELISA -- 6.Common questions and answers about ELISA.
520 $a This book offers comprehensive information on all aspects of ELISA, starting with the fundamentals of the immune system. It also reviews the history of analytical assays prior to the advent of ELISA (enzyme-linked immunosorbent assay) and addresses the materials of choice for the fabrication of the platforms, possible biomolecular interactions, different protocols, and evaluation parameters. The book guides readers through the respective steps of the analytical assay, while also familiarizing them with the possible sources of error in the assay. It offers detailed insights into the immobilization techniques used for protein attachment, as well as methods for evaluating the assay and calculating the key parameters, such as sensitivity, specificity, accuracy and limit of detection. In addition, the book explores the advantages and shortcomings of the conventional ELISA, as well as various approaches to improving its performance. In this regard, merging and integrating other technologies with widely known ELISAs have opened new avenues for the advancement of this immunoassay. Accordingly, the book provides cutting-edge information on integrated platforms such as ELISpot, plasmonic ELISAs, sphere-/bead-based ELISAs, paper-/fiber-based ELISAs and ELISA in micro-devices.
650 0 $a Enzyme-linked immunosorbent assay. $3 197413
650 1 4 $a Engineering. $3 210888
650 2 4 $a Biomedical Engineering. $3 190464
650 2 4 $a Immunology. $3 189152
650 2 4 $a Biological and Medical Physics, Biophysics. $3 759746
650 2 4 $a Protein-Ligand Interactions. $3 348845
650 2 4 $a Biotechnology. $3 188829
650 2 4 $a Computational Biology/Bioinformatics. $3 274833
700 1 $a Hosseini, Samira. $3 737906
710 2 $a SpringerLink (Online service) $3 273601
773 0 $t Springer eBooks
830 0 $a SpringerBriefs in applied sciences and technology. $3 557662
856 4 0 $u http://dx.doi.org/10.1007/978-981-10-6766-2
950 $a Engineering (Springer-11647)